abstract：:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...

journal_title：Research in microbiology

authors： Xu N,Qian K,Dong Y,Chen Y,Yu Q,Zhang B,Xing L,Li M

更新日期：2014-04-01 00:00:00

abstract：:The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. F...

journal_title：Research in microbiology

authors： Youenou B,Brothier E,Nazaret S

更新日期：2014-01-01 00:00:00

abstract：:The diversity and temporal dynamics of bacterial communities in pristine snow around two Russian Antarctic stations was investigated. Taxonomic analysis of rDNA libraries revealed that snow communities were dominated by bacteria from a small number of operational taxonomic units (OTUs) that underwent dramatic swings i...

journal_title：Research in microbiology

authors： Lopatina A,Krylenkov V,Severinov K

更新日期：2013-11-01 00:00:00

abstract：:Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...

journal_title：Research in microbiology

authors： Im S,Joe M,Kim D,Park DH,Lim S

更新日期：2013-11-01 00:00:00

abstract：:In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...

journal_title：Research in microbiology

authors： Kadouri DE,Shanks RM

更新日期：2013-10-01 00:00:00

abstract：:Sub-MIC antibiotics differentially modulate transcription of subsets of genes by unknown mechanisms. Paradoxically, the RNA polymerase inhibitor rifampicin is able to both upmodulate as well as downmodulate transcription when present at sub-MIC levels. In this study, we analyzed DNA sequences required for transcriptio...

journal_title：Research in microbiology

authors： Yim G,Spiegelman GB,Davies JE

更新日期：2013-06-01 00:00:00

abstract：:Sequences of the most conserved proteins encoded by transposable (pro)phages were used to search recently sequenced Firmicute genomes for candidate transposable prophages. One complete Mu-like prophage, SglyMu-1, was identified, in four copies, in the Syntrophobotulus glycolicus DSM 8271 chromosome sequence. Related p...

journal_title：Research in microbiology

authors： Toussaint A

更新日期：2013-05-01 00:00:00

abstract：:In the genus Pseudomonas, two main lineages and 19 phylogenetic groups or subgroups have been defined based on DNA sequence studies of 16S rRNA, gyrB and rpoD genes. In the present study, 33 strains previously classified as Pseudomonas putida were phylogenetically affiliated with their closest relatives in the genus b...

journal_title：Research in microbiology

authors： Mulet M,García-Valdés E,Lalucat J

更新日期：2013-05-01 00:00:00

abstract：:Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishin...

journal_title：Research in microbiology

authors： Decat E,Van Mechelen E,Saerens B,Vermeulen SJ,Boekhout T,De Blaiser S,Vaneechoutte M,Deschaght P

更新日期：2013-02-01 00:00:00

abstract：:Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs i...

journal_title：Research in microbiology

authors： Rotem O,Biran D,Ron EZ

更新日期：2013-01-01 00:00:00

abstract：:Explosives such as octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) provide a challenge in terms of bioremediation. In the present study, sheep rumen was studied for its potential to detoxify HMX using analytical chemistry and molecular microbial ecology tools. Results indicated significant loss (p < 0.05) of HM...

journal_title：Research in microbiology

authors： Perumbakkam S,Craig AM

更新日期：2012-09-01 00:00:00

abstract：:Metagenomic libraries represent subsamples of the total DNA found at a study site and offer unprecedented opportunities to study ecological and functional aspects of microbial communities. To examine the depth of a community sequencing effort, rarefaction analysis of the ribosomal small subunit (SSU/16S/18S) gene in t...

journal_title：Research in microbiology

authors： Bengtsson J,Hartmann M,Unterseher M,Vaishampayan P,Abarenkov K,Durso L,Bik EM,Garey JR,Eriksson KM,Nilsson RH

更新日期：2012-07-01 00:00:00

abstract：:Fungal dimorphism is the capacity of certain species of fungi to grow in the form of budding yeasts or mycelium depending on the environmental conditions. This characteristic is a complex phenomenon that involves modifications of the molecular machinery in response to different environmental signals. Through the use o...

journal_title：Research in microbiology

authors： Morales-Vargas AT,Domínguez A,Ruiz-Herrera J

更新日期：2012-06-01 00:00:00

abstract：:Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...

journal_title：Research in microbiology

authors： Park SY,Park SH,Choi SK

更新日期：2012-05-01 00:00:00

abstract：:Sequence analysis of the 16S ribosomal RNA gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. Our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which Actinobacteria, Prot...

journal_title：Research in microbiology

authors： Talia P,Sede SM,Campos E,Rorig M,Principi D,Tosto D,Hopp HE,Grasso D,Cataldi A

更新日期：2012-04-01 00:00:00

abstract：:In this study, the occurrence and diversity of integrons were evaluated in 697 isolates belonging to Enterobacteriaceae and Aeromonas spp. isolated from urban wastewaters. Screening of integrons was performed by dot blot hybridization and intI-positive strains were further characterized. The global prevalence of integ...

journal_title：Research in microbiology

authors： Moura A,Pereira C,Henriques I,Correia A

更新日期：2012-02-01 00:00:00

abstract：:Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of...

journal_title：Research in microbiology

authors： Bui XT,Wolff A,Madsen M,Bang DD

更新日期：2012-01-01 00:00:00

abstract：:The atmospheric concentration of methane (CH(4)), a major greenhouse gas, is mainly controlled by the activities of methane-producing (methanogens) and methane-consuming (methanotrophs) microorganisms. Freshwater lakes are identified as one of the main CH(4) sources, as it was estimated that they contribute to 6-16% o...

journal_title：Research in microbiology

authors： Borrel G,Jézéquel D,Biderre-Petit C,Morel-Desrosiers N,Morel JP,Peyret P,Fonty G,Lehours AC

更新日期：2011-11-01 00:00:00

abstract：:Bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. In this study, the prevalence of B. cereus bacteriophages in Korean fermented foods was studied using 6 reference strains, and JBP901 was further characterized. In a total of 47 samples, ...

journal_title：Research in microbiology

authors： Shin H,Bandara N,Shin E,Ryu S,Kim KP

更新日期：2011-10-01 00:00:00

abstract：:Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...

journal_title：Research in microbiology

authors： Pérez-Montaño F,Guasch-Vidal B,González-Barroso S,López-Baena FJ,Cubo T,Ollero FJ,Gil-Serrano AM,Rodríguez-Carvajal MÁ,Bellogín RA,Espuny MR

更新日期：2011-09-01 00:00:00

abstract：:Mrd1 is one of the trans-acting proteins and plays an important role in precursor ribosomal RNA processing. Here we characterized the Mamrd1 gene from Metarhizium acridum and studied its function in growth, conidiation and virulence using RNA interference. The Mamrd1 gene was identified as participating in the process...

journal_title：Research in microbiology

authors： Cao Y,Li K,Xia Y

更新日期：2011-09-01 00:00:00

abstract：:The association between bacteria and leaves in Ardisia has been described as a cyclic and obligate symbiosis in which bacteria are maintained throughout all stages of the plant's life cycle to guarantee normal growth and survival of the host. This intimate interaction suggests that both partners have co-diversified to...

journal_title：Research in microbiology

authors： Lemaire B,Smets E,Dessein S

更新日期：2011-06-01 00:00:00

abstract：:Bordetella isolates in the Saint Petersburg region have been monitored since 1998. Over the past ten years, concomitant with the increase in pertussis whole-cell vaccine coverage, the incidence of whooping cough has decreased. However, this decrease exists only for Bordetella pertussis infections, as the incidence of ...

journal_title：Research in microbiology

authors： Kurova N,Njamkepo E,Brun D,Tseneva G,Guiso N

更新日期：2010-12-01 00:00:00

abstract：:Bacterial membranes are known to form domains with specific lipid compositions and functions. Recently, using membrane binding fluorescent dyes, lipid spiral structures extending along the long axis of the cell were detected. These spirals were absent when the synthesis of phosphatidylglycerol and cardiolipin was disr...

journal_title：Research in microbiology

authors： Muchová K,Jamroškovič J,Barák I

更新日期：2010-11-01 00:00:00

abstract：:Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. T...

journal_title：Research in microbiology

authors： Biéler S,Silva F,Belin D

更新日期：2010-10-01 00:00:00

abstract：:Ecologists have documented the process of plant succession for centuries, yet the successional patterns exhibited by microbial communities have received relatively little attention. We examine recent work on microbial succession and show how, despite some key differences, studies of plant succession can serve as a tem...

journal_title：Research in microbiology

authors： Fierer N,Nemergut D,Knight R,Craine JM

更新日期：2010-10-01 00:00:00

abstract：:The phylogenetic diversity of a sediment microbial community from two ponds having different salinities, 150-200 g/l (M2) and 250-300 g/l (TS38), of an Sfax (Tunisia) solar saltern, was investigated using 16S rRNA clone libraries. The 16S rRNA genes from 135 bacterial clones and 105 archaeal clones were sequenced and ...

journal_title：Research in microbiology

authors： Baati H,Guermazi S,Gharsallah N,Sghir A,Ammar E

更新日期：2010-09-01 00:00:00

abstract：:The structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of the fruit tree pathogen Erwinia amylovora, was modeled based on predicted structural homology to characterized T3SS chaperones. This model guided the selection of 11 amino acid residues that were individually mutated to alani...

journal_title：Research in microbiology

authors： Triplett LR,Wedemeyer WJ,Sundin GW

更新日期：2010-09-01 00:00:00

abstract：:Soil is one of the most complex and challenging environments for microbiologists. In fact, although it contains the largest microbial diversity on the planet, the majority of these microbes are still uncharacterized and represent an enormous unexplored reservoir of genetic and metabolic diversity. Metagenomics, the st...

journal_title：Research in microbiology

authors： Mocali S,Benedetti A

更新日期：2010-07-01 00:00:00

abstract：:Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...

journal_title：Research in microbiology

authors： Santos C,Caetano T,Ferreira S,Mendo S

更新日期：2010-05-01 00:00:00

abstract：:A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...

journal_title：Research in microbiology

authors： Barbati S,Grenga L,Luzi G,Paolozzi L,Ghelardini P

更新日期：2010-03-01 00:00:00

abstract：:Some pathogenic species belonging to the Vibrionaceae family have been shown to regulate virulence through a complicated network of quorum sensing systems. In this study, three kinds of N-acylhomoserine lactone (AHL) signal molecules were detected in Vibrio ichthyoenteri DA3, a pathogen of cultured turbot (Scophthalmu...

journal_title：Research in microbiology

authors： Li X,Han Y,Yang Q,Zhang XH

更新日期：2010-01-01 00:00:00

abstract：:The work described here continues our studies of the effects of subinhibitory concentrations of antibiotics on SOS and DNA repair gene expression in Staphylococcus aureus. Mitomycin C and the new-generation fluoroquinolone moxifloxacin induced expression of SOS response genes (lexA, recA, sosA, and umuC) in a ciproflo...

journal_title：Research in microbiology

authors： Mesak LR,Davies J

更新日期：2009-12-01 00:00:00

abstract：:Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differential...

journal_title：Research in microbiology

authors： Santi L,Silva WO,Pinto AF,Schrank A,Vainstein MH

更新日期：2009-12-01 00:00:00

abstract：:Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...

journal_title：Research in microbiology

authors： Kupka D,Liljeqvist M,Nurmi P,Puhakka JA,Tuovinen OH,Dopson M

更新日期：2009-12-01 00:00:00

abstract：:Streptococcus intermedius, an oral commensal and a cause of systemic pyogenic disease, expresses fimbriae. To identify the gene(s) encoding these fimbriae, we used a serum raised against purified fimbriae to screen libaries of recombinant lambda phages. The cloned gene cluster encoding S. intermedius fimbriae, (saliva...

journal_title：Research in microbiology

authors： Yamaguchi T,Matsumoto M,Sugimoto Y,Soutome S,Oho T

更新日期：2009-12-01 00:00:00

abstract：:General and regulated proteolysis in bacteria is crucial for cellular homeostasis and relies on high substrate specificity of the executing AAA+ proteases. Here we summarize the various strategies that tightly control substrate degradation from both sides: the generation of accessible degrons and their specific recogn...

journal_title：Research in microbiology

authors： Schmidt R,Bukau B,Mogk A

更新日期：2009-11-01 00:00:00

abstract：:Clostridium tyrobutyricum is an anaerobic bacterium responsible for late blowing defects during cheese ripening and it is of scientific interest for biological hydrogen production. A scanning electron microscopy (SEM) coating technique and X-ray microanalysis were developed to analyze the architecture and chemical com...

journal_title：Research in microbiology

authors： Bassi D,Cappa F,Cocconcelli PS

更新日期：2009-06-01 00:00:00

abstract：:Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...

journal_title：Research in microbiology

authors： Tattevin P,Basuino L,Chambers HF

更新日期：2009-04-01 00:00:00

abstract：:The tricarballylate utilization locus (tcuRABC) of Salmonella enterica serovar Typhimurium is comprised of a 3-gene operon (tcuABC) that encodes functions that allow this bacterium to use tricarballylate as a source of carbon and energy, and the tcuR gene, which encodes a putative LysR-type transcriptional regulator. ...

journal_title：Research in microbiology

authors： Lewis JA,Stamper LW,Escalante-Semerena JC

更新日期：2009-04-01 00:00:00